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<h2>Building of a plot</h2>
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The following graphics explain the priciple of building of a siRNA hit plot:

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<img style="width: 1091px; height: 767px;" alt="" src="plot2.bmp">

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<h3>Plot window</h3>
<img style="width: 1084px; height: 585px;" alt="" src="plot.bmp">
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The plot shows that  the query sequence has two potential RNAi targets in the chosen database. The query match perfectly (100% identity) to the first target (Contig4), which leads to typical trapezoidal distribution of the total siRNA hits (blue line) with a maximum of 21 siRNA per position.  The maximum can be higher then 21 in case of highly repetitive sequence.
The second hit (U35_24651) is a potential off target regardless the absence of efficient siRNA hits. To improve the specificity of the RNAi the first ~300 nt of the trigger sequences should be omitted from the construct.
 The red line shows how many of the total siRNA match users definition of efficient siRNA given in the field "Parameters for defining efficient siRNA.


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